Targeting biopsy for a non-enhancing tumour. Conventional MRI: a, b Post-contrast T1W and T2W sequences demonstrate a large non-enhancing space occupying mass lesion without significant oedema. Multiparametric MRI: c, d Heterogeneous ADC and rCBV values throughout the lesion. e Multi-voxel MRS clearly shows focal area of very high Cho/Cr (3.1) and very small lactate peak. f Targeted biopsy taken from the area of highest choline peak (arrow). Histopathology shows anaplastic astrocytoma with moderately atypical astrocytic cells in a fibrillary background with a few abnormal mitoses (WHO grade III).